Lawson Laboratory Ltd
Ultrasonic DNA Shearing Device,Sonication Device,LAWSON20-A
Ultrasonic DNA Shearing Device,Sonication Device,LAWSON20-A
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Product Introduction
LAWSON20-A Non-contact ultrasonic DNA interrupter uses isothermal and non-contact methods to disrupt, homogenize, and mix samples. It is used for sterile, ultra-low-volume crushing and can break chromosomes through centrifuge tubes. It is specially tailored for pre-processing of second-generation sequencing DNA samples and chromatin immunoprecipitation experimental samples. Compared to traditional probe ultrasonic homogenizers, which directly contact the probe with the sample and can only process one sample at a time, resulting in a long experimental cycle. When dealing with thousands of samples, it is necessary to reuse the same probe, which can easily cause sample cross-contamination. The non-contact sample can be crushed in a closed container without producing infectious aerosols. The ultrasonic probe does not contact the sample, avoiding cross-contamination. For laboratories that process multiple samples per day or handle valuable samples, non-contact ultrasonic DNA homogenizers have the advantages of high throughput, low sample loss, and no cross-contamination. It has gradually become an indispensable standardized tool for ChlP (chromatin immunoprecipitation) and DNA cutting research platforms.
Product Features
⒈ No aerosol float produced - enhanced biological safety (e.g. branch bacteria, viruses, etc.)
⒉ Eliminates the risk of sample cross-contamination
⒊ Eliminates the traditional problem of probe wear and debris
⒋ Can process a wide range of samples with a broad sample processing range
⒌ Suitable for various standard containers
⒍ Can be used for processing trace samples, as small as 5ul
⒎ The automatic continuous rotation of the centrifuge tube makes the energy distribution of the ultrasonic wave more uniform
⒏ Optional high and low temperature constant temperature water bath, customized rotating base for Eppendorf tubes of various diameters according to customer needs
Experimental Results
Experimental purpose: Fragmentation of collected cell DNA samples: 4T1 cells derived from mouse mammary gland cancer. Instrument: Non-contact ultrasonic DNA fragmentation instrument.
Experimental data presentation of ultrasonic gene disruptor
| Experimental objective: |
| 1. Test the disruption time of different fragment sizes under the breaking condition of 50ul, 100% power (300W), ultrasonic time: 10s, interval time: 10s. |
| 2. Verify the repeatability and uniformity of disruption effect under the same disruption condition. |
| Test the effect of ultrasonic disruption damage on the yield of the product recovery. |
Application Range
| 1. Fragmentation of DNA samples for second-generation sequencing. |
| 2. Fragmentation of RNA. |
| 3. Disruption of bacteria and cells. |
| 4. ChIP assay (chromatin immunoprecipitation). |
| 5. Sample preparation for high-throughput sequencing instruments. |
| 6. Extraction of membrane proteins and other proteins. |
| 7. Homogenization and emulsification reactions. |
| 8. Ultrasonic treatment of expensive reagents. |
| 9. Immunoprecipitation experiments and catalytic reactions. |
Technical Specifications
| Model: | LAWSON20-A single-channel | LAWSON30-A dual-channel |
| Power: | 10-1000W continuously adjustable | 25-2500W continuously adjustable |
| Sample processing quantity: | 30*0.1ml, 20*0.65ml, 11*2ml and 5 5-15ml centrifuge tubes | 60*0.1ml, 40*0.65ml, 22*2ml and 10 5-15ml centrifuge tubes |
| Sonication sample volume: | 2ml or more, or less than 5µL | 2ml or more, or less than 5µL |
| Single sonication time: | 0.1-99.9S | 0.1-99.9S |
| Single interval time: | 0.1-99.9S | 0.1-99.9S |
| Total time (sonication+interval): | 1-99H59M59S | 1-99H59M59S |
| Frequency range: | 20-40KHz | 20-40KHz |
| Cooling system - temperature control range: | Portably constant temperature bath host (compressor cooling) 2~40℃ | Portably constant temperature bath host (compressor cooling) 2~40℃ |
| Temperature reading accuracy: | ±0.1 ℃ | ±0.1 ℃ |
| Compressor power: | 600W | 600W |
| Ultrasonic tank volume: | 15*14*10cm | 23*14*10cm |
| Adapter material: | 316 stainless steel material | 316 stainless steel material |
| Noise level: | <55dB | <55dB |
| Imported original multi-frequency ultrasonic transducer: | 3 sets | 6 sets |
| Power supply (optional 110V): | 220V/110V/ 50Hz/60HZ | 220V/110V/ 50Hz/60HZ |
