Lawson Laboratory Ltd
Ultrasonic DNA Shearing Device,Sonication Device,LAWSON20-A
Ultrasonic DNA Shearing Device,Sonication Device,LAWSON20-A
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Product Introduction
LAWSON20-A Non-contact ultrasonic DNA interrupter uses isothermal and non-contact methods to disrupt, homogenize, and mix samples. It is used for sterile, ultra-low-volume crushing and can break chromosomes through centrifuge tubes. It is specially tailored for pre-processing of second-generation sequencing DNA samples and chromatin immunoprecipitation experimental samples. Compared to traditional probe ultrasonic homogenizers, which directly contact the probe with the sample and can only process one sample at a time, resulting in a long experimental cycle. When dealing with thousands of samples, it is necessary to reuse the same probe, which can easily cause sample cross-contamination. The non-contact sample can be crushed in a closed container without producing infectious aerosols. The ultrasonic probe does not contact the sample, avoiding cross-contamination. For laboratories that process multiple samples per day or handle valuable samples, non-contact ultrasonic DNA homogenizers have the advantages of high throughput, low sample loss, and no cross-contamination. It has gradually become an indispensable standardized tool for ChlP (chromatin immunoprecipitation) and DNA cutting research platforms.
Product Features
⒈ No aerosol float produced - enhanced biological safety (e.g. branch bacteria, viruses, etc.)
⒉ Eliminates the risk of sample cross-contamination
⒊ Eliminates the traditional problem of probe wear and debris
⒋ Can process a wide range of samples with a broad sample processing range
⒌ Suitable for various standard containers
⒍ Can be used for processing trace samples, as small as 5ul
⒎ The automatic continuous rotation of the centrifuge tube makes the energy distribution of the ultrasonic wave more uniform
⒏ Optional high and low temperature constant temperature water bath, customized rotating base for Eppendorf tubes of various diameters according to customer needs
Experimental Results
Experimental purpose: Fragmentation of collected cell DNA samples: 4T1 cells derived from mouse mammary gland cancer. Instrument: Non-contact ultrasonic DNA fragmentation instrument.
Experimental data presentation of ultrasonic gene disruptor
| Experimental objective: |
| 1. Test the disruption time of different fragment sizes under the breaking condition of 50ul, 100% power (300W), ultrasonic time: 10s, interval time: 10s. |
| 2. Verify the repeatability and uniformity of disruption effect under the same disruption condition. |
| Test the effect of ultrasonic disruption damage on the yield of the product recovery. |
Application Range
| 1. Fragmentation of DNA samples for second-generation sequencing. |
| 2. Fragmentation of RNA. |
| 3. Disruption of bacteria and cells. |
| 4. ChIP assay (chromatin immunoprecipitation). |
| 5. Sample preparation for high-throughput sequencing instruments. |
| 6. Extraction of membrane proteins and other proteins. |
| 7. Homogenization and emulsification reactions. |
| 8. Ultrasonic treatment of expensive reagents. |
| 9. Immunoprecipitation experiments and catalytic reactions. |
Note
The results may vary depending on the sample type and operating conditions.
This instrument is intended for Research Use Only (RUO) and is not for diagnostic or human therapeutic use.
Technical Specifications
| Model: | LAWSON20-A single-channel | LAWSON30-A dual-channel |
| Power: | 10-1000W continuously adjustable | 25-2500W continuously adjustable |
| Sample processing quantity: | 30*0.1ml, 20*0.65ml, 11*2ml and 5 5-15ml centrifuge tubes | 60*0.1ml, 40*0.65ml, 22*2ml and 10 5-15ml centrifuge tubes |
| Sonication sample volume: | 2ml or more, or less than 5µL | 2ml or more, or less than 5µL |
| Single sonication time: | 0.1-99.9S | 0.1-99.9S |
| Single interval time: | 0.1-99.9S | 0.1-99.9S |
| Total time (sonication+interval): | 1-99H59M59S | 1-99H59M59S |
| Frequency range: | 20-40KHz | 20-40KHz |
| Cooling system - temperature control range: | Portably constant temperature bath host (compressor cooling) 2~40℃ | Portably constant temperature bath host (compressor cooling) 2~40℃ |
| Temperature reading accuracy: | ±0.1 ℃ | ±0.1 ℃ |
| Compressor power: | 600W | 600W |
| Ultrasonic tank volume: | 15*14*10cm | 23*14*10cm |
| Adapter material: | 316 stainless steel material | 316 stainless steel material |
| Noise level: | <55dB | <55dB |
| Imported original multi-frequency ultrasonic transducer: | 3 sets | 6 sets |
| Power supply (optional 110V): | 220V/110V/ 50Hz/60HZ | 220V/110V/ 50Hz/60HZ |
